GARDASIL AND MULTIPLE SCLEROSIS. BORRELIA GARINII IS THE CAUSE OF FAROE ISLANDS MULTIPLE SCLEROSIS-LIKE LYME DISEASE

Association pour la Recherche sur les Tumeurs de la Prostate (ARTP)

18 Nov 2015 . Palais des Congrès, Porte Maillot, Paris

TRAN Guy Mong Ky

Retired, Public Health (Agence Régionale de Santé Auvergne Rhône-Alpes), Hospital Hôtel Dieu, Clermont-Ferrand, FRANCE. Correspond: 31 Avenue du Bois 92290 Chatenay Malabry. E-mail:   This e-mail address is being protected from spambots. You need JavaScript enabled to view it . Phone:+33 9 81 89 38 70.

BACKGROUND Multiple sclerosis (MS)-like demyelinating encephalomyelitis was reported after Gardasil vaccination. Marie Oceane Bourguignon, a very severe case of post-Gardasil MS, was infected by Epstein-Barr Virus (EBV) with EBNA-1 antibodies. The best model of MS was the outbreak in Faroe Islands, with British Troops dogs. Borrelia can infect dogs. The ANSM September 2015 report was flawed by a bad protocole which arbitrarily excluded the 3^rd dose vaccinated patients for a third of patients, the auto-immune prone patients and those who have received vaccine injection before the start of the protocole. Namely, all the MS-like were excluded.

METHODS We compared the amino acid (AA) sequences of the 9 HPV (types 16, 18, 6, 11, 31, 33, 45, 52, 59) L1 with Myelin Basic Protein (MBP). The CerebroSpinal Fluid (CSF) epitope in Faroe MS (Rohowsky-Kochan C, 1995) was compared to Borrelia Garinii Far04 (Far=Faroe).

RESULTS L1 of HPV-, HPV-11,-6  is homologous to MBP [citrullinated R induces a fulminant MS (Wood DD, 1996)] encephalitogenic in the monkey (Karkhanis YD, 1975), to Borrelia Garinii, to canarypox (arrest of anti-Aids vaccine for myelitis) (Tran MKG, 2005), to vaccinia virus 42K-like  (Jahnke U,1985), to measles virus (SSPE encephalitis), to Theiler's virus capside, to EBV BRLF1

There is a very strong molecular homology of 15 AA length between Borrelia Garinii Far04 valyl tRNA synthetase, the epitope found in Cerebro Spinal Fluid (CSF) of Faroe Islands MS patients and encephalitogenic MBP (Whitaker JN, 1990) read in retro inverso.

This implicated very clearly Borrelia (Lyme disease) and not Canine Distemper Virus (Cook SD) as the culprit of Faroe Islands MS outbreak. Puffin ticks are the Borrelia reservoir. A review of the historical links between Lyme disease and MS is available on Vaughter S. owndoc.com. Blewett M, 2006, 2012.

CONCLUSION We propose Citrulline (pumpkin soup) as an alicament of MS. Gardasil may be dangerous in patients infected by EBV or Borrelia Burgdorferii sensu lato (B. Garinii). In Germany, there is 2 millions of patients infected by Borrelia and the epidemics continues to grow. Chronic neuroborreliosis is very difficult to diagnose and is generally overlooked, because the current test is completely unliable (35% sensibility) and prone to very frequent false negative errors. The correct test must be antigenically complete, supplied by a very specialized competent laboratory (Shaller V). The treatment is long with violent Herxheimer's reaction and antibiotics (Doxycycline, Rocephine, Azithromycine) must be supplemented by vitamin C [acerola (Raymond P)], Flagyl (to dissolve the kysts), colloidal silver and detoxification (Chronimed). Co-infections (Babebiosis) must be detected and treated (Horowitz R). Denmark must be particularly careful about Gardasil and now Gardasil 9, despite European Medicines Agency denial, because Denmark has endemic non diagnosed neuroborreliosis. Japan and India has stopped Gardasil

BIBLIOGRAPHY Albertat J. Maladie de Lyme (Thierry Souccar Ed.) 2012, 224 p. In French

Blewett M A Geostatistical Analysis of Possible Spirochetal Involvement in Multiple Sclerosis and Other Related Diseases. http://www.lymenet.de/literatur/Megan-Lyme_and_MS.htm. Blewett M, Kildluff M. 2012 Multiple Sclerosis geographics, investigating aetiology through space/time epidemiology. http://www.msgeographics.com/index_files/Page660.htm. Chronimed.over-blog.com [Montagnier L (Nobel Prize), Perrone C, Horowitz R, Raymond P)]. Cook SD. Epidemiological studies on multiple sclerosis. Neurology 1985, 35:1528-9. Fritzshe M Chronic Lyme borreliosis at the root of multiple sclerosis--is a cure with antibiotics attainable? Medical Hypotheses 2005;64(3):438-48. Horowitz R. Postface in: Albertat J. pp213-218. Horowitz R. Soigner Lyme et les maladies chroniques inexpliquées. Livre en français. Horowitz R. Maladie de Lyme : Mon parcours pour retrouver la santé 2012. Livre en français. Jahnke U. Sequence homology between certain viral proteins and proteins related to encephalomyelitis and neuritis. Science 1985,19: 282-4. Karkhanis YD. Allergic encephalomyelitis. Isolation of an encephalitogenic peptide active in the monkey. J Biol Chem 1975, 250: 1718-22. Raymond P. Stress oxydatif et infections froides. Http//chronimed.over-blog.com. Rohowsky-Kochan C. Canine distemper virus-specific antibodies in multiple sclerosis. Neurology 1995, 45: 1554-60. Shaller V Laboratory. 16, rue Oberlin. 67000 Strasbourg, France.  Tran MKG. Int AIDS Soc Conf 2005 (free on: Positifs.org). Vaughter S. Multiple sclerosis is Lyme disease: Anatomy of a cover-up. http://owndoc.com/lyme/multiple-sclerosis-is-lyme-disease-anatomy-of-a-cover-up. Wood DD. Acute multiple sclerosis (Marburg type) is associated with developmentally immature myelin basic protein. Ann Neurol 1996, 40:18-24.

Acknowlegments to Capriani A, President of the Association Positifs, positifs.org

Gardasil DANGER-Prise de Risques majeurs et Corruption

Le Dr Tran a soumis à l’ARTP 5 communications Poster à L’ARTP( association de recherche sur le cancer de la prostate) qui démontraient de manière irréfutable les raisons des effets indésirables  graves connus du vaccin Gardasil( laboratoire Merck), vaccin recommandé destiné à éviter les cancers liés au virus HPV( utérus, sein ,prostate,…) .Il s’agit de la stérilité définitive( génocide à terme des vaccinés) masquée par la prise de la pillule(C.98) , de l’encéphalopathie démyélinisante entrainant des  paralysies(C.99), et des hémorragies avec thrombopénie(C.100). Lors de l’ouverture  de la conférence , le Dr Tran a été agressé physiquement par 8 gardes  aux ordres du  Président de l’association, O. Cuvillier Les gardes ont déchiré ses Posters et emporté ses flyers qui étaient à disposition des participants. Le Président de l’association, O. Cuvillier, parfaitement informé des effets indésirables du vaccin s’est eclipsé pendant l’agression, puis est revenu  pour justifier l’exclusion du Dr Tran avec pour raison « Trouble à l’ordre public » !!!

Il s’agit d’une situation gravissime qui montre la collusion entre les laboratoires, le monde de la recherche et les  politiques responsables de la santé publique.

HUMAN PAPILLOMAVIRUS (HPV) AND PROSTATIC CANCER OSTEOBLASTIC BONE METASTASIS: HPV E6 CONTAINS AN ENDOTHELIN ACTIVE SITE TRYPTOPHAN

ARTP 18 Nov 2015 Paris Porte Maillot

TRAN Guy Mong Ky

Retired, Public Health (Agence Régionale de Santé ARS Auvergne Rhône Alpes), Hospital Hôtel-Dieu, Clermont-Ferrand, FRANCE. Correspondence: 31 Avenue du Bois 92290 Chatenay Malabry. E-mail: This e-mail address is being protected from spambots. You need JavaScript enabled to view it . Phone: +33 9 81 89 38 70.

BACKKROUND Endothelin (ET-1, 2, 3) (Yanagisawa M, 1989) is mitogenic for 3T3 fibroblasts (Takuwa N, 1989), induces osteoblast proliferation and is involved in osteoblastic bone metastases of Prostatic Cancer (PK) with translocation TMPRSS2:ERG gene fusion (Delliaux C, 2014). We found earlier by centering on the HQLL motif that:

HPV-18  E2           IQTLNHQVL

contains the osteoprotegerin active site           ET  SHQLL

as well as parathormone (PTH)                           IQ LMHNL

and  PTH-related Protein (PTHrP)                        S EHQLL

that could explain PK osteoclastic bone metastasis (Tran GMK, 2004). We search for an endothelin in HPV that could explain osteoblastic bone metastasis.

METHODS Amino acid sequences and three dimensional structure (3D) comparison between HPV and ET.

RESULTS By centering on the crucial tryptophan W21, which removal decreases ET potency by a factor > 1000 (Kimura S, 1988), we found that

HPV (cand 89), -150, -160 E6                  34-EL-35         48-LDIVW-52  is homologous to the

ET-1,-2,-3/sarafotoxin active site                5-DM-6          17-LDI IW-21

In 3D structure, HPV E6 has 2 separated functional sites, centered on the COOH-terminal tryptophane W 21 and on 5-DM-6 (of sarafotoxine). The homologous sequence LNVVW is found in HPV-3, -28, -29, -68 ME 180. In HPV-18 E6, W was replaced by Y. Burrowing asp Attractaspis engaddensis sarafotoxin, which induces a coronary spasm, differs slightly from ET:QDVIW (where Q replaced L).

Weak but highly selective ET-A Receptor antagonists were isolated from Streptomyces Misakiensis and sp n° 7338 (Miyata S, 1992). Highly potent and selective ET-A Receptor antagonist structures are pentapeptides:

cyclo (D-Asp-Pro-D-Val-Leu-D-Trp) (Ihara M, 1992) and

cyclo (D-Asp-Pro-D-Ile -Leu-D-Trp) (D=Dextrogyre) (Lippton, 1993).

CONCLUSION The molecular homology of HPV E6 with the active site of Endothelin, implicated in PK bone metastasis, supports further the HPV role in KP, not only in the primary tumor, but also specifically in osteoblastic bone metastasis. Interestingly, high doses of Vitamin C has an anti-Endothelin action (Dow C, 2015).

BIBLIOGRAPHY Delliaux C, Endothelin-1, a gene regulated by TMPRSS2:ERG fusion proteins in prostate cancer bone metastases. ARTP, Paris, Nov 19 2014. Ihara M. Biological profiles of highly potent novel endothelin antagonists selective for the ETA receptor. Life Sci 1992, 50: 247-55. Dow C.Vitamin C Supplementation Reduces ET-1 System Activity in Overweight and Obese Adults.14th Int Conf on Endothelin: Physiology, Pathophysiology and Therapeutics. Press release of the Am Physiol Soc, 4th September 2015, www.the-aps.org/mm/hp/Audiences/Public-Press/2015/44.html. Kimura S. Structure-activity relationships of endothelin: importance of the C-terminal moiety. Bio Bio Res Com 1988, 156: 1182-6. Miyata S. WS-7338, new endothelin receptor antagonists isolated from Streptomyces sp. No. 7338. I. Taxonomy, fermentation, isolation, physico-chemical properties and biological activities. J Antibiot (Tokyo) 1992, 45: 74-82; 83-7; 698-703;788-91; 1029-40; 1041-6. Lippton. Am Heart Assoc, 66^th Scientific Sessions, Georgia World Congress, Nov 8-11, 1993. Takuwa N. A novel vasoactive peptide endothelin stimulates mitogenesis through inositol lipid turnover in Swiss 3T3 fibroblasts. J Biol Chem 1989, 264: 7856-61. Tran GMK, Role of human papillomavirus type 18 in a subgroup of prostatic cancer with bone metastasis: Its protein E2 contains the osteoprotegerin active site EuroConf Cancer, Pasteur Inst. Jan 15-16 2004. Yanagisawa M. Molecular biology and biochemistry of the endothelins. Trends Pharmacol Sci 1989, 10: 374-8. Review.

PROSTATIC CANCER AND HUMAN PAPILLOMAVIRUS (HPV): MOLECULAR HOMOLOGY OF HPV-18 E2 WITH ONCOGENE CRIPTO-1.

Présenté à

ARTP 18 Nov 2015 Paris Porte Maillot

TRAN Guy Mong Ky

Retired, Public Health (Agence Régionale de Santé ARS Auvergne Rhône Alpes), Hospital Hôtel-Dieu, Clermont-Ferrand, FRANCE. Correspondence: 31 Avenue du Bois 92290 Chatenay Malabry. E-mail: This e-mail address is being protected from spambots. You need JavaScript enabled to view it . Phone: +33 9 81 89 38 70.

BACKROUND HPV are found in Prostatic Cancer (PK) by PCR only when the primer is E (=Early) and not L (=Late) (Tran GMK, 2004). This particularity may be explained by L loss during integration (Johansson H, 2013). Cripto-1 (Teratocarcinoma-Derived Growth Factor-1), a member of Epidermal Growth Factor (EGF) family, is involved in PK progression (Terry S, 2013, 2015). It is a co-receptor of TGF-β, induces E-Cadherin loss (Zhong XY, 2008) in Epithelial to Mesenchymal Transition (Thiery JP, 2002) and stimulates the PI3K/Akt pathway. E-Cadherin down regulation can also be induced by HPV E6 (Matthews K, 2003). As HPV contains many oncogenes implicated specifically in PK [HPV-11 E2 = EGF-HRG-TGFα mitogens chimera (Tran MKG, 1997) with a common MHIESL hexapeptide, Osteoprotegerin in bone metastases (Tran GMK, 2004), Nucleophosmin 1 (NPM1) in androgeno-regulation (Tran GMK, 2014)], we search for a molecular homology between Cripto-1 and HPV.

METHODS Amino acid sequences comparison of HPV with Cripto-1 EGF motif        

                                                             CcxxGGxCxxxxxCxCxxxxxxxxC.

RESULTS

HPV-18 E2                                   113-CFkKGGqTV 45-YFdGNK D 127-DGNKDNC

Cripto-1                                         83-CL

..

A      S                                           D W T L      323-PPNN   L  KCWR  Q RC    122-VQVYF

matches with Cripto-1                        120-D(W,T)L      124-LPKKcsLcKCWHgQlRC    142-PQAFL

CONCLUSION The discovery of Cripto-1 being a viral HPV-18 E2 oncogene, whereas EGF is a HPV-11 E2, confirms the HPV role in PK, and E-cadherin loss as a factor of metastasis. A safe and efficient vaccine is warranted, as Gardasil and Gardasil 9 based on L1 may be followed by menopause/sterility and  multiple sclerosis, and Cervarix by thrombocytopenia. A BT PHARMA vaccine based on a HPV-16 and -18 oncogene E7, instead of L1 as in Gardasil and Cervarix, is in phase II trial at the Pasteur Institute (Paris). E7 is much smaller than L1 and has less worrying epitopes.

BIBLIOGRAPHY Matthews K. Depletion of Langerhans cells in human papillomavirus type 16-infected skin is associated with E6-mediated down regulation of E-cadherin. J Virol 2003, 77: 8378-85. Terry S. Involvement of CRIPTO-1 in progression of prostate tumors. Eurocancer, Paris, June 25-26, 2013. Terry S. CRIPTO overexpression promotes mesenchymal differentiation in prostate carcinoma cells through parallel regulation of AKT and FGFR activities. Oncotarget 2015, 6: 11994-2008. Thiery JP.  Epithelial-mesenchymal transitions in tumour progression. Nat Rev Cancer 2002, 2: 442-54. Tran MKG. Human Papillomavirus (HPV) E2 protein contains a chimera of epidermal growth factor (EGF) and EGF family mitogens: Heregulin and Tumor Growth Factor. 6th Eur HIV Conf, Hamburg 1997, P474. Tran GMK. Role of human papillomavirus type 18 in a subgroup of prostatic cancer with bone metastasis: Its protein E2 contains the osteoprotegerin active site. EuroConf Cancer, Pasteur Inst. Jan 15-16 2004. Tran GMK. Prostatic cancer of viral origin: Homology of human oncogenic papillomavirus (HPV) L1 with nucleophosmin (NPM1), a controller of androgen receptor transcription. ARTP, Nov 19, 2014, Paris. Zhong XY. Positive association of up-regulated Cripto-1 and down-regulated E-cadherin with tumour progression and poor prognosis in gastric cancer. Histopathology. 2008, 52: 560-8.